Publications

Development of a monoclonal antibody to study MARCH6, an E3 ligase that regulates proteins that control lipid homeostasis.

Xu S, Donnelly L, Kober DL, Mak M, Radhakrishnan A, 2024 Sep J Lipid Res 100650

A cholesterol-binding bacterial toxin provides a strategy for identifying a specific Scap inhibitor that blocks lipid synthesis in animal cells.

Xu S, Smothers JC, Rye D, Endapally S, Chen H, Li S, Liang G, Kinnebrew M, Rohatgi R, Posner BA, Radhakrishnan A, 2024 Feb Proc Natl Acad Sci U S A 7 121 e2318024121

DGAT2 inhibition blocks SREBP-1 cleavage and improves hepatic steatosis by increasing phosphatidylethanolamine in the ER.

Rong S, Xia M, Vale G, Wang S, Kim CW, Li S, McDonald JG, Radhakrishnan A, Horton JD, 2024 Feb Cell Metab

A concerted mechanism involving ACAT and SREBPs by which oxysterols deplete accessible cholesterol to restrict microbial infection.

Heisler DB, Johnson KA, Ma DH, Ohlson MB, Zhang L, Tran M, Corley CD, Abrams ME, McDonald JG, Schoggins JW, Alto NM, Radhakrishnan A, 2023 Jan Elife 12

Measuring and Manipulating Membrane Cholesterol for the Study of Hedgehog Signaling.

Kinnebrew M, Johnson KA, Radhakrishnan A, Rohatgi R, 2022 Methods Mol Biol 2374 73-87

Patched 1 reduces the accessibility of cholesterol in the outer leaflet of membranes.

Kinnebrew M, Luchetti G, Sircar R, Frigui S, Viti LV, Naito T, Beckert F, Saheki Y, Siebold C, Radhakrishnan A, Rohatgi R, 2021 Oct Elife 10

Scap structures highlight key role for rotation of intertwined luminal loops in cholesterol sensing.

Kober DL, Radhakrishnan A, Goldstein JL, Brown MS, Clark LD, Bai XC, Rosenbaum DM, 2021 Jun Cell

The use of anthrolysin O and ostreolysin A to study cholesterol in cell membranes.

Johnson KA, Radhakrishnan A, 2021 Methods Enzymol 649 543-566

Identification of a degradation signal at the carboxy terminus of SREBP2: A new role for this domain in cholesterol homeostasis.

Kober DL, Xu S, Li S, Bajaj B, Liang G, Rosenbaum DM, Radhakrishnan A, 2020 Oct Proc Natl Acad Sci U S A

Accessible cholesterol is localized in bacterial plasma membrane protrusions.

Abrams ME, Johnson KA, Radhakrishnan A, Alto NM, 2020 Jul J. Lipid Res.

Cholesterol access in cellular membranes controls Hedgehog signaling.

Radhakrishnan A, Rohatgi R, Siebold C, 2020 Dec Nat Chem Biol 12 16 1303-1313

Accessibility of cholesterol at cell surfaces.

Johnson KA, Radhakrishnan A, 2020 Apr J. Lipid Res.

Oxysterols provide innate immunity to bacterial infection by mobilizing cell surface accessible cholesterol.

Abrams ME, Johnson KA, Perelman SS, Zhang LS, Endapally S, Mar KB, Thompson BM, McDonald JG, Schoggins JW, Radhakrishnan A, Alto NM, 2020 Apr Nat Microbiol

Cholesterol Stabilizes TAZ in Hepatocytes to Promote Experimental Non-alcoholic Steatohepatitis.

Wang X, Cai B, Yang X, Sonubi OO, Zheng Z, Ramakrishnan R, Shi H, Valenti L, Pajvani UB, Sandhu J, Infante RE, Radhakrishnan A, Covey DF, Guan KL, Buck J, Levin LR, Tontonoz P, Schwabe RF, Tabas I, 2020 Apr Cell Metab.

Ostreolysin A and anthrolysin O use different mechanisms to control movement of cholesterol from the plasma membrane to the endoplasmic reticulum.

Johnson KA, Endapally S, Vazquez DC, Infante RE, Radhakrishnan A, 2019 Oct J. Biol. Chem.

Cholesterol accessibility at the ciliary membrane controls Hedgehog signaling.

Kinnebrew M, Iverson EJ, Patel BB, Pusapati GV, Kong JH, Johnson KA, Luchetti G, Eckert KM, McDonald JG, Covey DF, Siebold C, Radhakrishnan A, Rohatgi R, 2019 Oct Elife 8

Molecular Discrimination between Two Conformations of Sphingomyelin in Plasma Membranes.

Endapally S, Frias D, Grzemska M, Gay A, Tomchick DR, Radhakrishnan A 2019 Jan Cell

Monitoring and Modulating Intracellular Cholesterol Trafficking Using ALOD4, a Cholesterol-Binding Protein.

Endapally S, Infante RE, Radhakrishnan A 2019 Methods Mol. Biol. 1949 153-163

Retrospective on Cholesterol Homeostasis: The Central Role of Scap

Brown MS, Radhakrishnan A, Goldstein JL 2018 Jun Annu Rev Biochem. 87 783-807

Variability of cholesterol accessibility in human red blood cells measured using a bacterial cholesterol-binding toxin.

Chakrabarti RS, Ingham SA, Kozlitina J, Gay A, Cohen JC, Radhakrishnan A, Hobbs HH 2017 Feb Elife 6

Continuous transport of a small fraction of plasma membrane cholesterol to endoplasmic reticulum regulates total cellular cholesterol.

Infante RE, Radhakrishnan A 2017 Apr Elife 6

Cholesterol-induced conformation changes in the sterol-sensing domain of the scap protein suggest feedback mechanism to control cholesterol synthesis.

Gao Y, Zhou Y, Goldstein JL, Brown MS, Radhakrishnan A 2017 Apr J. Biol. Chem.

Direct Demonstration that Loop1 of Scap Binds to Loop7, a crucial event in cholesterol homeostasis.

Zhang Y, Lee KM, Kinch LN, Clark L, Grishin NV, Rosenbaum DM, Brown MS, Goldstein JL, Radhakrishnan A 2016 Apr J. Biol. Chem.

Switch-like Responses of Two Cholesterol Sensors Do Not Require Protein Oligomerization in Membranes.

Gay A, Rye D, Radhakrishnan A 2015 Mar Biophys. J. 6 108 1459-69

PROTEIN STRUCTURE. Crystal structure of a mycobacterial Insig homolog provides insight into how these sensors monitor sterol levels.

Ren R, Zhou X, He Y, Ke M, Wu J, Liu X, Yan C, Wu Y, Gong X, Lei X, Yan SF, Radhakrishnan A, Yan N 2015 Jul Science 6244 349 187-91

Three pools of plasma membrane cholesterol and their relation to cholesterol homeostasis.

Das A, Brown MS, Anderson DD, Goldstein JL, Radhakrishnan A 2014 Jun Elife e02882

Use of mutant 125I-Perfringolysin O to probe transport and organization of cholesterol in membranes of animal cells.

Das A, Goldstein JL, Anderson DD, Brown MS, Radhakrishnan A 2013 Jun Proc. Natl. Acad. Sci. U.S.A. 26 110 10580-5

Accessibility of cholesterol in endoplasmic reticulum membranes and activation of SREBP-2 switch abruptly at a common cholesterol threshold.

Sokolov A, Radhakrishnan A 2010 Sep J. Biol. Chem. 38 285 29480-90