Petroll Lab

Cell Mechanics and Corneal Wound Healing

The Petroll Lab applies engineering approaches and design principles to the investigation of fundamental clinical and biological problems in ophthalmology, while providing training to graduate students, medical students, and postdocs.

Research

The main focus of the Petroll Lab’s research is cell mechanics and tissue engineering, whereby multidimensional time-lapse imaging is used to investigate how the mechanical behavior of corneal fibroblasts is regulated by both biochemical and biophysical stimuli. Our lab also has a longstanding interest in the development and application of in vivo confocal microscopy and in situ multiphoton imaging, which allow quantitative 3-D assessment of cell differentiation and extracellular matrix organization during corneal wound healing in response to clinical procedures such as refractive surgery or UV cross-linking.

applied research We develop and apply in vivo confocal microscopy and in situ multiphoton imaging techniques to assess the cellular responses to corneal injury, disease and refractive surgical procedures.

We use multidimensional time-lapse imaging to investigate how the behavior of corneal fibroblasts is regulated by biochemical and biophysical stimuli encountered during wound healing.

Male scientist showing two female grad students an image on a computer screen.
    Cell Mechanics

    Overview

    The organization of extracellular matrices by cells through the exertion of mechanical forces drives fundamental processes such as developmental morphogenesis, wound healing, and the organization of bioengineered tissues. Historically, our ability to investigate cell mechanical behavior has been limited by the technical challenges associated with measuring the sub-cellular origins of cellular force generation and local matrix patterning in a 3-D environment. Thus, our understanding of these fundamental processes is limited, especially in ocular tissues. Over the last several years, our research team has addressed these challenges through the development of new experimental models, use of emerging imaging technologies, and the application of quantitative analysis techniques. 

    Experimental Models

    Our in vitro approaches include: 1) models for assessing how specific growth factors expressed following injury or surgery alter the mechanical differentiation of quiescent corneal keratocytes within 3-D collagen matrices, 2) novel approaches for assessing the response of fibroblasts to dynamic changes in ECM mechanical properties (as might be observed during development or wound healing), 3) 3-D constructs for assessing the mechanical interplay between fibroblast migration, sub-cellular force generation, and ECM patterning, and 4) engineered substrates that allow investigation of the impact of ECM protein composition, topography, and elasticity on cell differentiation and mechanical behavior.

    Together, these experimental models are providing unique insights into the underlying biochemical and biomechanical signaling mechanisms controlling corneal fibroblast migration, contraction, and matrix reorganization. This is fundamental information which can not be obtained using standard 2-D culture models, and may eventually lead to improved strategies for modulating cell mechanical activity during wound healing, and for designing artificial matrices and directing cell behavior during corneal tissue engineering. 

    Corneal Wound Healing

    The cornea is an optically clear tissue that forms the front surface of the eye, and accounts for nearly two-thirds of its refractive power. The corneal stroma, which makes up 90% of corneal thickness, is a highly organized structure consisting of collagen lamellae with specific packing and spacing that is critical to maintenance of corneal transparency. Corneal stromal cells (keratocytes) reside between the collagen lamellae, and are responsible for secreting extracellular matrix (ECM) components required to maintain normal corneal structure and function (i.e. transparency). Because it is exposed, the cornea is susceptible to infection, physical and chemical injuries; it is also the target of many vision correction procedures.

    Stromal keratocytes play a central role in mediating the corneal response to lacerating injury, chemical injuries or surgical procedures. During wound healing, quiescent corneal keratocytes surrounding the area of injury generally become activated, and transform into a fibroblastic repair phenotype. In certain wound types, fibroblasts further differentiate into myofibroblasts, which generate stronger forces and synthesize a disorganized fibrotic ECM. Cellular force generation and fibrosis can alter corneal shape and/or reduce corneal transparency, thereby reducing visual acuity.

    In addition to fibrosis which develops on top of the wound bed, most vision correction procedures induce keratocyte death beneath the laser-treated area.  Stromal cell death can also be induced by toxic injury, as well as UV cross-linking (CXL) of the cornea in keratoconus patients. Ideally, repopulation following these insults should occur via intra-stromal migration of keratocytes from the surrounding stromal tissue, without generation of strong contractile forces that could disrupt the collagen architecture and lead to vision loss.

    Our lab develops and applies high resolution imaging and image processing approaches to better understand and potentially modulate these healing responses. Specifically, we use in vivo confocal microscopy to assess stromal keratocyte differentation, patterning and activation in response corneal injury, surgery, or disease. We also use 3-D confocal and multiphoton imaging of corneal tissue ex vivo to assess the expression and localization of specific proteins associated with wound healing (using fluorescent labeling) as well as changes in extracellular matrix organization (using second harmonic generation imaging).1,2

    References

    1. Petroll WM, Kivanany PB, Hagenasr D, Graham EK. Corneal Fibroblast Migration Patterns During Intrastromal Wound Healing Correlate With ECM Structure and Alignment. Invest Ophthalmol Vis Sci. 2015 Nov;56(12):7352-61.

    2. Kivanany PB, Grose KC, Tippani M, Su S, Petroll WM. Assessment of Corneal Stromal Remodeling and Regeneration after Photorefractive Keratectomy. Sci Rep. 2018 Aug 22;8(1):12580. 

    Video: About the Petroll Lab

    Meet the Principal Investigator

    Dr. Petroll in lab coat holding up and examining lab samples.

    W. Matthew Petroll, Ph.D.

    Dr. Petroll received his B.S. degree in Biomedical Engineering from Duke University in 1984, and his Ph.D. in Biomedical Engineering from the University of Virginia in 1989. After two years at Georgetown University in the lab of Drs. James Jester and Dwight Cavanagh, he joined the faculty at UT Southwestern in 1991. He was promoted to professor in 2005, and became Chair of the Graduate Program in Biomedical Engineering in 2012.

    Dr. Petroll’s laboratory applies engineering approaches and design principles to the investigation of fundamental clinical and biological problems in ophthalmology, while providing training to graduate students, medical students, and post-docs. The main focus of Dr. Petroll’s research is cell mechanics and tissue engineering, in which multidimensional time-lapse imaging is used to investigate how the mechanical behavior of corneal fibroblasts is regulated by both biochemical and biophysical stimuli. He also has a longstanding interest in the development and application of in vivo confocal microscopy, which allows quantitative 3-D imaging of the cornea and has been used in numerous research and clinical studies on corneal wound healing, toxicity, development and infection.

    View Faculty Profile

    Publications

    Keratocyte mechanobiology.

    Petroll WM, Varner VD, Schmidtke DW, 2020 Sep 200 108228

    Assessment of Corneal Stromal Remodeling and Regeneration after Photorefractive Keratectomy.

    Kivanany PB, Grose KC, Tippani M, Su S, Petroll WM 2018 Aug Sci Rep 1 8 12580

    Fibroblast-fibronectin patterning and network formation in 3D fibrin matrices.

    Miron-Mendoza M, Graham E, Manohar S, Petroll WM 2017 Jun Matrix Biol.

    Corneal Fibroblast Migration Patterns During Intrastromal Wound Healing Correlate With ECM Structure and Alignment.

    Petroll WM, Kivanany PB, Hagenasr D, Graham EK 2015 Nov Invest. Ophthalmol. Vis. Sci. 12 56 7352-61

    Techniques for assessing 3-D cell-matrix mechanical interactions in vitro and in vivo.

    Miron-Mendoza M, Koppaka V, Zhou C, Petroll WM 2013 Jun Exp. Cell Res.

    Cell motility and mechanics in three-dimensional collagen matrices.

    Grinnell F, Petroll WM 2010 Nov Annu. Rev. Cell Dev. Biol. 26 335-61

    Localized application of mechanical and biochemical stimuli in 3-D culture.

    Petroll WM, Ma L 2008 Oct Dev. Dyn. 10 237 2726-36

    Loss of Corneal Nerves and Corneal Haze in Patients with Fuchs' Endothelial Corneal Dystrophy with the Transcription Factor 4 Gene Trinucleotide Repeat Expansion.

    Gillings M, Mastro A, Zhang X, Kiser K, Gu J, Xing C, Robertson DM, Petroll WM, Mootha VV, 2023 Mar Ophthalmol Sci 1 3 100214

    Sutureless Conjunctiva-Sparing Posterior Ptosis Repair Surgery: A Novel Technique.

    Mancini R, Forouzan P, Keenum ZG, Tenzel PA, Petroll WM, 2023 Jul Am J Ophthalmol 251 77-89

    The impact of UV cross-linking on corneal stromal cell migration, differentiation and patterning.

    Petroll WM, Miron-Mendoza M, Sunkara Y, Ikebe HR, Sripathi NR, Hassaniardekani H, 2023 Aug Exp Eye Res 233 109523

    Effects of Topography and PDGF on the Response of Corneal Keratocytes to Fibronectin-Coated Surfaces.

    Lam KH, Shihabeddin TZ, Awkal JA, Najjar AM, Miron-Mendoza M, Maruri DP, Varner VD, Petroll WM, Schmidtke DW, 2023 Apr J Funct Biomater 4 14

    ECM stiffness modulates the proliferation but not the motility of primary corneal keratocytes in response to PDGF-BB.

    Iyer KS, Maruri DP, Peak KE, Schmidtke DW, Petroll WM, Varner VD, 2022 May Exp Eye Res 109112

    Temporal and Spatial Assessment of the Corneal Response to UV Cross-Linking Using 3-Dimensional In Vivo Confocal Microscopy.

    Parsa S, Rodriguez A, Robertson DM, Bowman RW, Petroll WM, 2022 Mar Eye Contact Lens

    Quercetin Decreases Corneal Haze In Vivo and Influences Gene Expression of TGF-Beta Mediators In Vitro.

    McKay TB, Kivanany PB, Nicholas SE, Nag OK, Elliott MH, Petroll WM, Karamichos D, 2022 Jul Metabolites 7 12

    Signaling Downstream of Focal Adhesions Regulates Stiffness-Dependent Differences in the TGF-?1-Mediated Myofibroblast Differentiation of Corneal Keratocytes.

    Maruri DP, Iyer KS, Schmidtke DW, Petroll WM, Varner VD, 2022 Front Cell Dev Biol 10 886759

    Infectious keratitis after corneal crosslinking: a systematic review.

    Murchison CE, Petroll WM, Robertson DM, 2021 Feb J Cataract Refract Surg

    Evaluation of the Repeatability of the LacryDiag Ocular Surface Analyzer for Assessment of the Meibomian Glands and Tear Film.

    Ward CD, Murchison CE, Petroll WM, Robertson DM, 2021 08 Transl Vis Sci Technol 9 10 1

    Fibulin-3 knockout mice demonstrate corneal dysfunction but maintain normal retinal integrity.

    Daniel S, Renwick M, Chau VQ, Datta S, Maddineni P, Zode G, Wade EM, Robertson SP, Petroll WM, Hulleman JD, 2020 Sep

    ECM Stiffness Controls the Activation and Contractility of Corneal Keratocytes in Response to TGF-?1.

    Maruri DP, Miron-Mendoza M, Kivanany PB, Hack JM, Schmidtke DW, Petroll WM, Varner VD, 2020 Sep Biophys J

    Outcomes of Resident-Performed Laser-Assisted vs Traditional Phacoemulsification.

    Hansen B, Blomquist PH, Ririe P, Pouly S, Nguyen C, Petroll WM, McCulley JP, 2020 May J Cataract Refract Surg

    Lower Corneal Haze and Aberrations in Descemet Membrane Endothelial Keratoplasty Versus Descemet Stripping Automated Endothelial Keratoplasty in Fellow Eyes for Fuchs Endothelial Corneal Dystrophy.

    Waldrop WH, Gillings MJ, Robertson DM, Petroll WM, Mootha VV, 2020 Jul Cornea

    Coupling of Fibrin Reorganization and Fibronectin Patterning by Corneal Fibroblasts in Response to PDGF BB and TGF?1.

    Miron-Mendoza M, Vazquez D, García-Rámila N, Ikebe HR, Petroll WM, 2020 Aug Bioengineering (Basel) 3 7

    Positive toxicology and reactive serology in tissue donors: a retrospective study over a 3-year period.

    Heck E, Gruslin K, Corder V, Petroll WM, Urban J, 2020 Apr Cell Tissue Bank

    A high-throughput microfluidic method for fabricating aligned collagen fibrils to study Keratocyte behavior.

    Lam KH, Kivanany PB, Grose K, Yonet-Tanyeri N, Alsmadi N, Varner VD, Petroll WM, Schmidtke DW, 2019 11 Biomed Microdevices 4 21 99

    An In Vitro Model for Assessing Corneal Keratocyte Spreading and Migration on Aligned Fibrillar Collagen.

    Kivanany PB, Grose KC, Yonet-Tanyeri N, Manohar S, Sunkara Y, Lam KH, Schmidtke DW, Varner VD, Petroll WM 2018 Sep J Funct Biomater 4 9

    Second harmonic generation imaging of corneal stroma after infection by Pseudomonas aeruginosa.

    Robertson DM, Rogers NA, Petroll WM, Zhu M 2017 Apr Sci Rep 7 46116

    Temporal and spatial analysis of stromal cell and extracellular matrix patterning following lamellar keratectomy.

    Kivanany PB, Grose KC, Petroll WM 2016 Oct Exp. Eye Res. 153 56-64

    Immunomodulatory therapy with anti-interleukin-1 after photorefractive keratectomy in the New Zealand white rabbit.

    Taylor KR, Panday VA, Caldwell MC, Petroll WM, Apsey DA, Reilly CD 2016 Jun J Cataract Refract Surg 6 42 913-9

    In vivo confocal microscopy of the cornea: New developments in image acquisition, reconstruction and analysis using the HRT-Rostock Corneal Module.

    Petroll WM, Robertson DM 2015 May Ocul Surf

    Impact of Donor Age on Corneal Endothelium-Descemet Membrane Layer Scroll Formation.

    Bennett A, Mahmoud S, Drury D, Cavanagh HD, McCulley JP, Petroll WM, Mootha VV 2015 Jan Eye Contact Lens

    TCF4 Triplet Repeat Expansion and Nuclear RNA Foci in Fuchs' Endothelial Corneal Dystrophy.

    Mootha VV, Hussain I, Cunnusamy K, Graham E, Gong X, Neelam S, Xing C, Kittler R, Petroll WM 2015 Feb Invest. Ophthalmol. Vis. Sci.

    Mechanical interactions and crosstalk between corneal keratocytes and the extracellular matrix.

    Petroll WM, Miron-Mendoza M 2015 Apr Exp. Eye Res. 133 49-57

    The Role of Thrombin and Cell Contractility in Regulating Clustering and Collective Migration of Corneal Fibroblasts in Different ECM Environments.

    Miron-Mendoza M, Graham E, Kivanany P, Quiring J, Petroll WM 2015 Invest. Ophthalmol. Vis. Sci. 3 56 2079-90

    Fibroblastic Transformation of Corneal Keratocytes by Rac Inhibition is Modulated by Extracellular Matrix Structure and Stiffness.

    Petroll WM, Lakshman N 2015 J Funct Biomater 2 6 222-40

    Effect of HDAC Inhibitors on Corneal Keratocyte Mechanical Phenotypes in 3-D Collagen Matrices.

    Koppaka V, Lakshman N, Petroll WM 2015 Mol. Vis. 21 502-14

    Pseudomonas aeruginosa Infectious Keratitis in a High Oxygen Transmissible Rigid Contact Lens Rabbit Model.

    Wei C, Zhu M, Petroll WM, Robertson DM 2014 Sep Invest. Ophthalmol. Vis. Sci. 9 55 5890-9

    MMP regulation of corneal keratocyte motility and mechanics in 3-D collagen matrices.

    Zhou C, Petroll WM 2014 Feb Exp. Eye Res.

    The Impact of Type 1 Diabetes Mellitus on Corneal Epithelial Nerve Morphology and the Corneal Epithelium.

    Cai D, Zhu M, Petroll WM, Koppaka V, Robertson DM 2014 Aug Am. J. Pathol.

    Cell-matrix interactions: New tools and fresh perspectives.

    Petroll WM, L Billiar K 2013 Aug Exp. Cell Res.

    Quantitative 3-Dimensional Corneal Imaging In Vivo Using a Modified HRT-RCM Confocal Microscope.

    Petroll WM, Weaver M, Vaidya S, McCulley JP, Cavanagh HD 2012 Oct Cornea

    Corneal epithelial cell viability following exposure to ophthalmic solutions containing preservatives and/or antihypertensive agents.

    Whitson JT, Petroll WM 2012 Oct Adv Ther 10 29 874-88

    Experimental Models for Investigating Intra-Stromal Migration of Corneal Keratocytes, Fibroblasts and Myofibroblasts.

    Petroll WM, Lakshman N, Ma L 2012 Mar J Funct Biomater 1 3 183-198

    Individual versus collective fibroblast spreading and migration: regulation by matrix composition in 3D culture.

    Miron-Mendoza M, Lin X, Ma L, Ririe P, Petroll WM 2012 Jun Exp. Eye Res. 99 36-44

    Corneal stromal cells use both high- and low-contractility migration mechanisms in 3-D collagen matrices.

    Kim A, Zhou C, Lakshman N, Petroll WM 2012 Apr Exp. Cell Res. 6 318 741-52

    Growth factor regulation of corneal keratocyte mechanical phenotypes in 3-D collagen matrices.

    Lakshman N, Petroll WM 2012 Invest. Ophthalmol. Vis. Sci. 3 53 1077-86

    Comparative study of descemet stripping automated endothelial keratoplasty donor preparation by Moria CBm microkeratome, horizon microkeratome, and Intralase FS60.

    Mootha VV, Heck E, Verity SM, Petroll WM, Lakshman N, Muftuoglu O, Bowman RW, McCulley JP, Cavanagh HD 2011 Mar Cornea 3 30 320-4

    A reconstituted telomerase-immortalized human corneal epithelium in vivo: a pilot study.

    Robertson DM, Kalangara JP, Baucom RB, Petroll WM, Cavanagh HD 2011 Aug Curr. Eye Res. 8 36 706-12

    Castroviejo Lecture 2009: 40 years in search of the perfect contact lens.

    Cavanagh HD, Robertson DM, Petroll WM, Jester JV 2010 Oct Cornea 10 29 1075-85

    Comparative Study of Descemet Stripping Automated Endothelial Keratoplasty Donor Preparation by Moria CBm Microkeratome, Horizon Microkeratome, and Intralase FS60.

    Mootha VV, Heck E, Verity SM, Petroll WM, Lakshman N, Muftuoglu O, Bowman RW, McCulley JP, Cavanagh HD 2010 Nov Cornea

    Rho Kinase Regulation of Fibroblast Migratory Mechanics in Fibrillar Collagen Matrices.

    Zhou C, Petroll WM 2010 Mar Cell Mol Bioeng 1 3 76-83

    Risk factors for intraocular pressure elevation after descemet stripping automated endothelial keratoplasty.

    Allen MB, Lieu P, Mootha VV, Bowman RW, Petroll WM, Tong L, Kooner KS, Cavanagh HD, Whitson JT, Aggarwal NK 2010 Jul Eye Contact Lens 4 36 223-7

    Inducible macropinocytosis of hyaluronan in B16-F10 melanoma cells.

    Greyner HJ, Wiraszka T, Zhang LS, Petroll WM, Mummert ME 2010 Jul Matrix Biol. 6 29 503-10

    Characterization of corneal keratocyte morphology and mechanical activity within 3-D collagen matrices.

    Lakshman N, Kim A, Petroll WM 2010 Feb Exp. Eye Res. 2 90 350-9

    Growth factor regulation of corneal keratocyte differentiation and migration in compressed collagen matrices.

    Kim A, Lakshman N, Karamichos D, Petroll WM 2010 Feb Invest. Ophthalmol. Vis. Sci. 2 51 864-75

    Remote-controlled scanning and automated confocal microscopy through focusing using a modified HRT rostock corneal module.

    Petroll WM, Cavanagh HD 2009 Nov Eye Contact Lens 6 35 302-8

    Intraocular pressure reduction in the untreated fellow eye after selective laser trabeculoplasty.

    Rhodes KM, Weinstein R, Saltzmann RM, Aggarwal N, Kooner KS, Petroll WM, Whitson JT 2009 Mar Curr Med Res Opin 3 25 787-96

    Outcomes of PermaVision intracorneal implants for the correction of hyperopia.

    Verity SM, McCulley JP, Bowman RW, Cavanagh HD, Petroll WM 2009 Jun Am. J. Ophthalmol. 6 147 973-7

    Development of a hyaluronan bioconjugate for the topical treatment of melanoma.

    Zhang LS, Greyner HJ, Mummert ME, Petroll WM 2009 Jul J. Dermatol. Sci. 1 55 56-9

    Tandem scanning confocal microscopy of cornea after descemet stripping automated endothelial keratoplasty.

    Prasher P, Muftuoglu O, Bowman RW, McCulley JP, Petroll WM, Cavanagh HD, Mootha VV 2009 Jul Eye Contact Lens 4 35 196-202

    An experimental model for assessing fibroblast migration in 3-D collagen matrices.

    Karamichos D, Lakshman N, Petroll WM 2009 Jan Cell Motil. Cytoskeleton 1 66 1-9

    Dynamic assessment of fibroblast mechanical activity during Rac-induced cell spreading in 3-D culture.

    Petroll WM, Ma L, Kim A, Ly L, Vishwanath M 2008 Oct J. Cell. Physiol. 1 217 162-71

    Assessment of keratocyte activation following LASIK with flap creation using the IntraLase FS60 laser.

    Petroll WM, Bowman RW, Cavanagh HD, Verity SM, Mootha VV, McCulley JP 2008 Oct J Refract Surg 8 24 847-9

    Development of a hyaluronan targeted contrast reagent for the demarcation of melanoma margins in vivo.

    Rudrabhatla SR, Petroll WM, Mahaffey CL, Mummert ME 2008 Mar J. Invest. Dermatol. 3 128 740-2

    Analysis of the pattern of subcellular force generation by corneal fibroblasts after Rho activation.

    Petroll WM, Ma L, Ly L, Vishwanath M 2008 Jan Eye Contact Lens 1 34 65-70

    The effect of nonpreserved care solutions on 12 months of daily and extended silicone hydrogel contact lens wear.

    Robertson DM, Petroll WM, Cavanagh HD 2008 Jan Invest. Ophthalmol. Vis. Sci. 1 49 7-15

    Quantitative assessment of corneal wound healing following IntraLASIK using in vivo confocal microscopy.

    McCulley JP, Petroll WM 2008 Trans Am Ophthalmol Soc 106 84-90; discussion 90-2

    Postnatal corneal transparency, keratocyte cell cycle exit and expression of ALDH1A1.

    Jester JV, Lee YG, Huang J, Houston J, Adams B, Cavanagh HD, Petroll WM 2007 Sep Invest. Ophthalmol. Vis. Sci. 9 48 4061-9

    Insulin-like Growth Factor Binding Protein-3 expression in the human corneal epithelium.

    Robertson DM, Ho SI, Hansen BS, Petroll WM, Cavanagh HD 2007 Oct Exp. Eye Res. 4 85 492-501

    The role of contact lens type, oxygen transmission, and care-related solutions in mediating epithelial homeostasis and pseudomonas binding to corneal cells: an overview.

    Robertson DM, Petroll WM, Jester JV, Cavanagh HD 2007 Nov Eye Contact Lens 6 Pt 2 33 394-8; discussion 399-400

    Regulation of corneal fibroblast morphology and collagen reorganization by extracellular matrix mechanical properties.

    Karamichos D, Lakshman N, Petroll WM 2007 Nov Invest. Ophthalmol. Vis. Sci. 11 48 5030-7

    Current concepts: contact lens related Pseudomonas keratitis.

    Robertson DM, Petroll WM, Jester JV, Cavanagh HD 2007 May Cont Lens Anterior Eye 2 30 94-107

    Rho plays a central role in regulating local cell-matrix mechanical interactions in 3D culture.

    Lakshman N, Kim A, Bayless KJ, Davis GE, Petroll WM 2007 Jun Cell Motil. Cytoskeleton 6 64 434-45

    Updates in Acanthamoeba keratitis.

    Awwad ST, Petroll WM, McCulley JP, Cavanagh HD 2007 Jan Eye Contact Lens 1 33 1-8

    Severe reactive ischemic posterior segment inflammation in acanthamoeba keratitis: a new potentially blinding syndrome.

    Awwad ST, Heilman M, Hogan RN, Parmar DN, Petroll WM, McCulley JP, Cavanagh HD 2007 Feb Ophthalmology 2 114 313-20

    Slit-lamp, confocal, and light microscopic findings of corneal siderosis.

    Witherspoon SR, Hogan RN, Petroll WM, Mootha VV 2007 Dec Cornea 10 26 1270-2

    Local thermal injury elicits immediate dynamic behavioural responses by corneal Langerhans cells.

    Ward BR, Jester JV, Nishibu A, Vishwanath M, Shalhevet D, Kumamoto T, Petroll WM, Cavanagh HD, Takashima A 2007 Apr Immunology 4 120 556-72

    Comparison of the corneal response to laser in situ keratomileusis with flap creation using the FS15 and FS30 femtosecond lasers: clinical and confocal microscopy findings.

    Hu MY, McCulley JP, Cavanagh HD, Bowman RW, Verity SM, Mootha VV, Petroll WM 2007 Apr J Cataract Refract Surg 4 33 673-81

    Prospective evaluation of PermaVision intracorneal implants using in vivo confocal microscopy.

    Lindsey SS, McCulley JP, Cavanagh HD, Verity SM, Bowman RW, Petroll WM 2007 Apr J Refract Surg 4 23 410-3

    Dynamic assessment of cell-matrix mechanical interactions in three-dimensional culture.

    Petroll WM 2007 Methods Mol. Biol. 370 67-82

    Assessment of corneal epithelial integrity after acute exposure to ocular hypotensive agents preserved with and without benzalkonium chloride.

    Whitson JT, Cavanagh HD, Lakshman N, Petroll WM 2006 Sep-Oct Adv Ther 5 23 663-71

    Differential interference contrast and confocal reflectance imaging of collagen organization in three-dimensional matrices.

    Petroll WM 2006 Nov-Dec Scanning 6 28 305-10

    Quantitative assessment of local collagen matrix remodeling in 3-D culture: the role of Rho kinase.

    Kim A, Lakshman N, Petroll WM 2006 Nov Exp. Cell Res. 18 312 3683-92

    Noninvasive corneal stromal collagen imaging using two-photon-generated second-harmonic signals.

    Morishige N, Petroll WM, Nishida T, Kenney MC, Jester JV 2006 Nov J Cataract Refract Surg 11 32 1784-91

    Prolonged hypoxia induces lipid raft formation and increases Pseudomonas internalization in vivo after contact lens wear and lid closure.

    Yamamoto N, Yamamoto N, Jester JV, Petroll WM, Cavanagh HD 2006 May Eye Contact Lens 3 32 114-20

    Evaluation of intrastromal lipid deposits after intacs implantation using in vivo confocal microscopy.

    Ly LT, McCulley JP, Verity SM, Cavanagh HD, Bowman RW, Petroll WM 2006 Jul Eye Contact Lens 4 32 211-5

    Confocal assessment of the effects of fourth-generation fluoroquinolones on the cornea.

    Ly LT, Cavanagh HD, Petroll WM 2006 Jul Eye Contact Lens 4 32 161-5

    Confocal assessment of the corneal response to intracorneal lens insertion and laser in situ keratomileusis with flap creation using IntraLase.

    Petroll WM, Goldberg D, Lindsey SS, Kelley PS, Cavanagh HD, Bowman RW, Parmar DN, Verity SM, McCulley JP 2006 Jul J Cataract Refract Surg 7 32 1119-28

    Bcl-2 and Bax regulation of corneal homeostasis in genetically altered mice.

    Robertson DM, Ladage PM, Yamamoto N, Jester JV, Petroll WM, Cavanagh HD 2006 Jan Eye Contact Lens 1 32 3-7

    Tandem scanning confocal corneal microscopy in the diagnosis of suspected acanthamoeba keratitis.

    Parmar DN, Awwad ST, Petroll WM, Bowman RW, McCulley JP, Cavanagh HD 2006 Apr Ophthalmology 4 113 538-47

    Central corneal thickness in patients with congenital aniridia.

    Whitson JT, Liang C, Godfrey DG, Petroll WM, Cavanagh HD, Patel D, Fellman RL, Starita RJ 2005 Sep Eye Contact Lens 5 31 221-4

    Quantitative assessment of ophthalmic viscosurgical device retention using in vivo confocal microscopy.

    Petroll WM, Jafari M, Lane SS, Jester JV, Cavanagh HD 2005 Dec J Cataract Refract Surg 12 31 2363-8

    Corneal fibroblasts respond rapidly to changes in local mechanical stress.

    Petroll WM, Vishwanath M, Ma L 2004 Oct Invest. Ophthalmol. Vis. Sci. 10 45 3466-74

    Pseudomonas aeruginosa corneal binding after 24-hour orthokeratology lens wear.

    Ladage PM, Yamamoto N, Robertson DM, Jester JV, Petroll WM, Cavanagh HD 2004 Jul Eye Contact Lens 3 30 173-8

    Dynamic three-dimensional visualization of collagen matrix remodeling and cytoskeletal organization in living corneal fibroblasts.

    Petroll WM, Cavanagh HD, Jester JV 2004 Jan-Feb Scanning 1 26 1-10

    Four-dimensional multiphoton confocal microscopy: the new frontier in cellular imaging.

    Jester JV, Ward BR, Takashima A, Gatlin J, Garcia JV, Cavanagh HD, Petroll WM 2004 Jan Ocul Surf 1 2 10-20

    Evaluation of the corneal effects of topical ophthalmic fluoroquinolones using in vivo confocal microscopy.

    Kovoor TA, Kim AS, McCulley JP, Cavanagh HD, Jester JV, Bugde AC, Petroll WM 2004 Apr Eye Contact Lens 2 30 90-4

    Modulation of corneal fibroblast contractility within fibrillar collagen matrices.

    Vishwanath M, Ma L, Otey CA, Jester JV, Petroll WM 2003 Nov Invest. Ophthalmol. Vis. Sci. 11 44 4724-35

    Effects of eyelid closure and disposable and silicone hydrogel extended contact lens wear on rabbit corneal epithelial proliferation.

    Ladage PM, Ren DH, Petroll WM, Jester JV, Bergmanson JP, Cavanagh HD 2003 May Invest. Ophthalmol. Vis. Sci. 5 44 1843-9

    In vivo fluorescent labeling of corneal wound healing fibroblasts.

    Gatlin J, Melkus MW, Padgett A, Petroll WM, Cavanagh HD, Garcia JV, Jester JV 2003 Mar Exp. Eye Res. 3 76 361-71

    Vertical movement of epithelial basal cells toward the corneal surface during use of extended-wear contact lenses.

    Ladage PM, Jester JV, Petroll WM, Bergmanson JP, Cavanagh HD 2003 Mar Invest. Ophthalmol. Vis. Sci. 3 44 1056-63

    Effects of daily and overnight wear of hyper-oxygen transmissible rigid and silicone hydrogel lenses on bacterial binding to the corneal epithelium: 13-month clinical trials.

    Cavanagh HD, Ladage P, Yamamoto K, Li SL, Petroll WM, Jester JV 2003 Jan Eye Contact Lens 1 Suppl 29 S14-6; discussion S26-9, S192-4

    Role of oxygen in corneal epithelial homeostasis during extended contact lens wear.

    Ladage PM, Jester JV, Petroll WM, Bergmanson JP, Cavanagh HD 2003 Jan Eye Contact Lens 1 Suppl 29 S2-6; discussion S26-9, S192-4

    Effects of contact lens care solutions on surface exfoliation and bacterial binding to corneal epithelial cells.

    Li SL, Ladage PM, Yamamoto T, Petroll WM, Jester JV, Cavanagh HD 2003 Jan Eye Contact Lens 1 29 27-30

    Can postlens tear thickness be measured using three-dimensional in vivo confocal microscopy?

    Petroll WM, Kovoor T, Ladage PM, Cavanagh HD, Jester JV, Robertson DM 2003 Jan Eye Contact Lens 1 Suppl 29 S110-4; discussion S115-8, S192-4

    Neonatal corneal stromal development in the normal and lumican-deficient mouse.

    Song J, Lee YG, Houston J, Petroll WM, Chakravarti S, Cavanagh HD, Jester JV 2003 Feb Invest. Ophthalmol. Vis. Sci. 2 44 548-57

    Effect of ophthalmic viscosurgical devices on lens epithelial cells: a morphological study.

    Budo C, Goffinet G, Bellotto D, Petroll WM 2003 Dec J Cataract Refract Surg 12 29 2411-8

    Direct, dynamic assessment of cell-matrix interactions inside fibrillar collagen lattices.

    Petroll WM, Ma L 2003 Aug Cell Motil. Cytoskeleton 4 55 254-64

    Direct correlation of collagen matrix deformation with focal adhesion dynamics in living corneal fibroblasts.

    Petroll WM, Ma L, Jester JV 2003 Apr J. Cell. Sci. Pt 8 116 1481-91

    Recovery time of corneal epithelial proliferation in the rabbit following rigid gas-permeable extended contact-lens wear.

    Ladage PM, Yamamoto K, Ren DH, Jester JV, Petroll WM, Bergmanson JP, Cavanagh HD 2003 Apr Eye Contact Lens 2 29 61-4

    Spherical indentations of human and rabbit corneal epithelium following extended contact lens wear.

    Ladage PM, Petroll WM, Jester JV, Fisher S, Bergmanson JP, Cavanagh HD 2002 Oct CLAO J 4 28 177-80

    Effects of daily and overnight wear of a novel hyper oxygen-transmissible soft contact lens on bacterial binding and corneal epithelium: a 13-month clinical trial.

    Cavanagh HD, Ladage PM, Li SL, Yamamoto K, Molai M, Ren DH, Petroll WM, Jester JV 2002 Nov Ophthalmology 11 109 1957-69

    Corneal epithelial homeostasis following daily and overnight contact lens wear.

    Ladage PM, Yamamoto K, Li L, Ren DH, Petroll WM, Jester JV, Cavanagh HD 2002 Mar Cont Lens Anterior Eye 1 25 11-21

    In vivo confocal microscopy through-focusing to measure corneal flap thickness after laser in situ keratomileusis.

    Gokmen F, Jester JV, Petroll WM, McCulley JP, Cavanagh HD 2002 Jun J Cataract Refract Surg 6 28 962-70

    A prototype two-detector confocal microscope for in vivo corneal imaging.

    Petroll WM, Yu A, Li J, Jester JV, Cavanagh HD, Black T 2002 Jul-Aug Scanning 4 24 163-70

    Intraocular pressure measurement after hyperopic LASIK.

    Wang X, Shen J, McCulley JP, Bowman RW, Petroll WM, Cavanagh HD 2002 Jul CLAO J 3 28 136-9

    Regulation of endotoxin-induced keratitis by PECAM-1, MIP-2, and toll-like receptor 4.

    Khatri S, Lass JH, Heinzel FP, Petroll WM, Gomez J, Diaconu E, Kalsow CM, Pearlman E 2002 Jul Invest. Ophthalmol. Vis. Sci. 7 43 2278-84

    Annexin V binding to rabbit corneal epithelial cells following overnight contact lens wear or eyelid closure.

    Li L, Ren DH, Ladage PM, Yamamoto K, Petroll WM, Jester JV, Cavanagh HD 2002 Jan CLAO J 1 28 48-54

    Effect of eyelid closure and overnight contact lens wear on viability of surface epithelial cells in rabbit cornea.

    Yamamoto K, Ladage PM, Ren DH, Li L, Petroll WM, Jester JV, Cavanagh HD 2002 Jan Cornea 1 21 85-90

    Adaptive effects of 30-night wear of hyper-O(2) transmissible contact lenses on bacterial binding and corneal epithelium: a 1-year clinical trial.

    Ren DH, Yamamoto K, Ladage PM, Molai M, Li L, Petroll WM, Jester JV, Cavanagh HD 2002 Jan Ophthalmology 1 109 27-39; discussion 39-40

    TGFbeta induced myofibroblast differentiation of rabbit keratocytes requires synergistic TGFbeta, PDGF and integrin signaling.

    Jester JV, Huang J, Petroll WM, Cavanagh HD 2002 Dec Exp. Eye Res. 6 75 645-57

    Effects of O2 transmissibility on corneal epithelium after daily and extended contact lens wear in rabbit and man.

    Ladage PM, Yamamoto K, Li L, Ren DH, Petroll WM, Jester JV, Cavanagh HD 2002 Adv. Exp. Med. Biol. Pt B 506 885-93

    Proliferation rate of rabbit corneal epithelium during overnight rigid contact lens wear.

    Ladage PM, Yamamoto K, Ren DH, Li L, Jester JV, Petroll WM, Bergmanson JP, Cavanagh HD 2001 Nov Invest. Ophthalmol. Vis. Sci. 12 42 2804-12

    Pathology of ocular irritation with bleaching agents in the rabbit low-volume eye test.

    Maurer JK, Molai A, Parker RD, Li L, Carr GJ, Petroll WM, Cavanagh HD, Jester JV 2001 May-Jun Toxicol Pathol 3 29 308-19

    Pathology of ocular irritation with acetone, cyclohexanol, parafluoroaniline, and formaldehyde in the rabbit low-volume eye test.

    Maurer JK, Molai A, Parker RD, Li LI, Carr GJ, Petroll WM, Cavanagh HD, Jester JV 2001 Mar-Apr Toxicol Pathol 2 29 187-99

    Effects of low and hyper Dk rigid gas permeable contact lenses on Bcl-2 expression and apoptosis in the rabbit corneal epithelium.

    Yamamoto K, Ladage PM, Ren DH, Li L, Petroll WM, Jester JV, Cavanagh HD 2001 Jul CLAO J 3 27 137-43

    Effects of rigid and soft contact lens daily wear on corneal epithelium, tear lactate dehydrogenase, and bacterial binding to exfoliated epithelial cells.

    Ladage PM, Yamamoto K, Ren DH, Li L, Jester JV, Petroll WM, Cavanagh HD 2001 Jul Ophthalmology 7 108 1279-88

    Corneal haze after photorefractive keratectomy using different epithelial removal techniques: mechanical debridement versus laser scrape.

    Lee YG, Chen WY, Petroll WM, Cavanagh HD, Jester JV 2001 Jan Ophthalmology 1 108 112-20

    Organization of junctional proteins in proliferating cat corneal endothelium during wound healing.

    Petroll WM, Ma L, Jester JV, Cavanagh HD, Bean J 2001 Jan Cornea 1 20 73-80

    Bcl-2 expression in the human cornea.

    Yamamoto K, Ladage PM, Ren DH, Li L, Petroll WM, Jester JV, Cavanagh HD 2001 Aug Exp. Eye Res. 2 73 247-55

    Measurement of corneal sublayer thickness and transparency in transgenic mice with altered corneal clarity using in vivo confocal microscopy.

    Jester JV, Ghee Lee Y, Li J, Chakravarti S, Paul J, Petroll WM, Dwight Cavanagh H 2001 Vision Res. 10-11 41 1283-90

    Quantitative characterization of acid- and alkali-induced corneal injury in the low-volume eye test.

    Jester JV, Molai A, Petroll WM, Parker RD, Carr GJ, Cavanagh HD, Maurer JK 2000 Sep-Oct Toxicol Pathol 5 28 668-78

    Specular microscopy, confocal microscopy, and ultrasound biomicroscopy: diagnostic tools of the past quarter century.

    Cavanagh HD, El-Agha MS, Petroll WM, Jester JV 2000 Sep Cornea 5 19 712-22

    On-line 3-dimensional confocal imaging in vivo.

    Li J, Jester JV, Cavanagh HD, Black TD, Petroll WM 2000 Sep Invest. Ophthalmol. Vis. Sci. 10 41 2945-53

    Corneal opacity in lumican-null mice: defects in collagen fibril structure and packing in the posterior stroma.

    Chakravarti S, Petroll WM, Hassell JR, Jester JV, Lass JH, Paul J, Birk DE 2000 Oct Invest. Ophthalmol. Vis. Sci. 11 41 3365-73

    Laser in situ keratomileusis flap margin: wound healing and complications imaged by in vivo confocal microscopy.

    Vesaluoma MH, Petroll WM, Pérez-Santonja JJ, Valle TU, Alió JL, Tervo TM 2000 Nov Am. J. Ophthalmol. 5 130 564-73

    Confocal microscopy of a patient with irregular astigmatism after LASIK reoperations and relaxation incisions.

    Linna TU, Vesaluoma MH, Petroll WM, Tarkkanen AH, Tervo TM 2000 Mar Cornea 2 19 163-9

    In vivo confocal microscopy of patients with corneal recurrent erosion syndrome or epithelial basement membrane dystrophy.

    Rosenberg ME, Tervo TM, Petroll WM, Vesaluoma MH 2000 Mar Ophthalmology 3 107 565-73

    Autosomal recessive cornea plana: in vivo corneal morphology and corneal sensitivity.

    Vesaluoma MH, Sankila EM, Gallar J, Müller LJ, Petroll WM, Moilanen JA, Forsius H, Tervo TM 2000 Jul Invest. Ophthalmol. Vis. Sci. 8 41 2120-6

    Stromal wound healing explains refractive instability and haze development after photorefractive keratectomy: a 1-year confocal microscopic study.

    Moller-Pedersen T, Cavanagh HD, Petroll WM, Jester JV 2000 Jul Ophthalmology 7 107 1235-45

    Effect of myopic LASIK on corneal sensitivity and morphology of subbasal nerves.

    Linna TU, Vesaluoma MH, Pérez-Santonja JJ, Petroll WM, Alió JL, Tervo TM 2000 Feb Invest. Ophthalmol. Vis. Sci. 2 41 393-7

    Corneal stromal changes induced by myopic LASIK.

    Vesaluoma M, Pérez-Santonja J, Petroll WM, Linna T, Alió J, Tervo T 2000 Feb Invest. Ophthalmol. Vis. Sci. 2 41 369-76

    Effect of cell migration on the maintenance of tension on a collagen matrix.

    Roy P, Petroll WM, Chuong CJ, Cavanagh HD, Jester JV 1999 Nov-Dec Ann Biomed Eng 6 27 721-30

    Changes in corneal endothelial apical junctional protein organization after corneal cold storage.

    Hsu JK, Cavanagh HD, Jester JV, Ma L, Petroll WM 1999 Nov Cornea 6 18 712-20

    Corneal stromal wound healing in refractive surgery: the role of myofibroblasts.

    Jester JV, Petroll WM, Cavanagh HD 1999 May Prog Retin Eye Res 3 18 311-56

    The cellular basis of corneal transparency: evidence for 'corneal crystallins'.

    Jester JV, Moller-Pedersen T, Huang J, Sax CM, Kays WT, Cavangh HD, Petroll WM, Piatigorsky J 1999 Mar J. Cell. Sci. 112 ( Pt 5) 613-22

    The effect of rigid gas permeable contact lens wear on proliferation of rabbit corneal and conjunctival epithelial cells.

    Ren DH, Petroll WM, Jester JV, Cavanagh HD 1999 Jul CLAO J 3 25 136-41

    Quantitative measurement of acute corneal injury in rabbits with surfactants of different type and irritancy.

    Maurer JK, Parker RD, Petroll WM, Carr GJ, Cavanagh HD, Jester JV 1999 Jul Toxicol. Appl. Pharmacol. 1 158 61-70

    Labeling of cycling corneal endothelial cells during healing with a monoclonal antibody to the Ki67 antigen (MIB-1).

    Petroll WM, Jester JV, Bean J, Cavanagh HD 1999 Jan Cornea 1 18 98-108

    The spatial organization of apical junctional complex-associated proteins in feline and human corneal endothelium.

    Petroll WM, Hsu JK, Bean J, Cavanagh HD, Jester JV 1999 Jan Curr. Eye Res. 1 18 10-9

    Transforming growth factor(beta)-mediated corneal myofibroblast differentiation requires actin and fibronectin assembly.

    Jester JV, Huang J, Barry-Lane PA, Kao WW, Petroll WM, Cavanagh HD 1999 Aug Invest. Ophthalmol. Vis. Sci. 9 40 1959-67

    The relationship between contact lens oxygen permeability and binding of Pseudomonas aeruginosa to human corneal epithelial cells after overnight and extended wear.

    Ren DH, Petroll WM, Jester JV, Ho-Fan J, Cavanagh HD 1999 Apr CLAO J 2 25 80-100

    Short-term hypoxia downregulates epithelial cell desquamation in vivo, but does not increase Pseudomonas aeruginosa adherence to exfoliated human corneal epithelial cells.

    Ren DH, Petroll WM, Jester JV, Ho-Fan J, Cavanagh HD 1999 Apr CLAO J 2 25 73-9

    Exertion of tractional force requires the coordinated up-regulation of cell contractility and adhesion.

    Roy P, Petroll WM, Cavanagh HD, Jester JV 1999 Cell Motil. Cytoskeleton 1 43 23-34

    Measurement of tissue thickness using confocal microscopy.

    Jester JV, Petroll WM, Cavanagh HD 1999 Meth. Enzymol. 307 230-45

    Characterization of specular "dark events" in human donor corneal endothelium by scanning and transmission electron microscopy.

    Nartey IN, Cavanagh HD, Jester JV, Andrews P, Petroll WM 1998 Sep Cornea 5 17 544-9

    Myofibroblast transformation of cat corneal endothelium by transforming growth factor-beta1, -beta2, and -beta3.

    Petroll WM, Jester JV, Bean JJ, Cavanagh HD 1998 Oct Invest. Ophthalmol. Vis. Sci. 11 39 2018-32

    Corneal haze development after PRK is regulated by volume of stromal tissue removal.

    Møller-Pedersen T, Cavanagh HD, Petroll WM, Jester JV 1998 Nov Cornea 6 17 627-39

    Area and depth of surfactant-induced corneal injury correlates with cell death.

    Jester JV, Li HF, Petroll WM, Parker RD, Cavanagh HD, Carr GJ, Smith B, Maurer JK 1998 May Invest. Ophthalmol. Vis. Sci. 6 39 922-36

    Confocal microscopic characterization of wound repair after photorefractive keratectomy.

    Møller-Pedersen T, Li HF, Petroll WM, Cavanagh HD, Jester JV 1998 Mar Invest. Ophthalmol. Vis. Sci. 3 39 487-501

    Assessment of stress fiber orientation during healing of radial keratotomy wounds using confocal microscopy.

    Petroll WM, Cavanagh HD, Jester JV 1998 Mar Scanning 2 20 74-82

    Neutralizing antibody to TGFbeta modulates stromal fibrosis but not regression of photoablative effect following PRK.

    Møller-Pedersen T, Cavanagh HD, Petroll WM, Jester JV 1998 Jul Curr. Eye Res. 7 17 736-47

    Area and depth of surfactant-induced corneal injury predicts extent of subsequent ocular responses.

    Jester JV, Petroll WM, Bean J, Parker RD, Carr GJ, Cavanagh HD, Maurer JK 1998 Dec Invest. Ophthalmol. Vis. Sci. 13 39 2610-25

    Clinical confocal microscopy.

    Petroll WM, Cavanagh HD, Jester JV 1998 Aug Curr Opin Ophthalmol 4 9 59-65

    Inhibition of corneal fibrosis by topical application of blocking antibodies to TGF beta in the rabbit.

    Jester JV, Barry-Lane PA, Petroll WM, Olsen DR, Cavanagh HD 1997 Mar Cornea 2 16 177-87

    Epithelial and corneal thickness measurements by in vivo confocal microscopy through focusing (CMTF).

    Li HF, Petroll WM, Møller-Pedersen T, Maurer JK, Cavanagh HD, Jester JV 1997 Mar Curr. Eye Res. 3 16 214-21

    Quantification of stromal thinning, epithelial thickness, and corneal haze after photorefractive keratectomy using in vivo confocal microscopy.

    Møller-Pedersen T, Vogel M, Li HF, Petroll WM, Cavanagh HD, Jester JV 1997 Mar Ophthalmology 3 104 360-8

    Adherence of Pseudomonas aeruginosa to shed rabbit corneal epithelial cells after overnight wear of contact lenses.

    Ren H, Petroll WM, Jester JV, Cavanagh HD, Mathers WD, Bonanno JA, Kennedy RH 1997 Jan CLAO J 1 23 63-8

    Characterization of SV40-transfected cell strains from rabbit keratocytes.

    Barry-Lane PA, Wilson SE, Cavanagh HD, Petroll WM, Jester JV 1997 Jan Cornea 1 16 72-8

    ZO-1 reorganization and myofibroblast transformation of corneal endothelial cells after freeze injury in the cat.

    Petroll WM, Barry-Lane PA, Cavanagh HD, Jester JV 1997 Feb Exp. Eye Res. 2 64 257-67

    Collagen fiber architecture of a cultured dermal tissue.

    Sacks MS, Chuong CJ, Petroll WM, Kwan M, Halberstadt C 1997 Feb J Biomech Eng 1 119 124-7

    An in vitro force measurement assay to study the early mechanical interaction between corneal fibroblasts and collagen matrix.

    Roy P, Petroll WM, Cavanagh HD, Chuong CJ, Jester JV 1997 Apr Exp. Cell Res. 1 232 106-17

    Confocal microscopic characterization of initial corneal changes of surfactant-induced eye irritation in the rabbit.

    Maurer JK, Li HF, Petroll WM, Parker RD, Cavanagh HD, Jester JV 1997 Apr Toxicol. Appl. Pharmacol. 2 143 291-300

    Effects of basic FGF and TGF beta 1 on F-actin and ZO-1 organization during cat endothelial wound healing.

    Petroll WM, Jester JV, Barry-Lane PA, Cavanagh HD 1996 Sep Cornea 5 15 525-32

    Induction of alpha-smooth muscle actin expression and myofibroblast transformation in cultured corneal keratocytes.

    Jester JV, Barry-Lane PA, Cavanagh HD, Petroll WM 1996 Sep Cornea 5 15 505-16

    In vivo confocal microscopy: a new possibility to confirm the diagnosis of Borrelia keratitis?

    Linna T, Mikkilä H, Karma A, Seppälä I, Petroll WM, Tervo T 1996 Nov Cornea 6 15 639-40

    Computational models of the effects of hydration on corneal biomechanics and the results of radial keratotomy.

    Roy P, Petroll WM, McKinney AE, Chuong CJ 1996 May J Biomech Eng 2 118 255-8

    Measurement of surgically induced corneal deformations using three-dimensional confocal microscopy.

    Petroll WM, Roy P, Chuong CJ, Hall B, Cavanagh HD, Jester JV 1996 Mar Cornea 2 15 154-64

    Application of in vivo confocal microscopy to the understanding of surfactant-induced ocular irritation.

    Jester JV, Maurer JK, Petroll WM, Wilkie DA, Parker RD, Cavanagh HD 1996 Jul-Aug Toxicol Pathol 4 24 412-28

    Quantitative three-dimensional confocal imaging of the cornea in situ and in vivo: system design and calibration.

    Petroll WM, Jester JV, Cavanagh HD 1996 Jan Scanning 1 18 45-9

    In vivo confocal imaging.

    Petroll WM, Jester JV, Cavanagh HD 1996 Int Rev Exp Pathol 36 93-129

    Assessment of f-actin organization and apical-basal polarity during in vivo cat endothelial wound healing.

    Petroll WM, Jester JV, Barry-Lane P, Cavanagh HD 1995 Nov Invest. Ophthalmol. Vis. Sci. 12 36 2492-502

    The spatial organization of corneal endothelial cytoskeletal proteins and their relationship to the apical junctional complex.

    Barry PA, Petroll WM, Andrews PM, Cavanagh HD, Jester JV 1995 May Invest. Ophthalmol. Vis. Sci. 6 36 1115-24

    Quantitative assessment of anteroposterior keratocyte density in the normal rabbit cornea.

    Petroll WM, Boettcher K, Barry P, Cavanagh HD, Jester JV 1995 Jan Cornea 1 14 3-9

    Basic science and applications of in vivo microscopy.

    Mathers WD, Petroll WM, Jester JV, Cavanagh HD 1995 Aug Curr Opin Ophthalmol 4 6 86-94

    Expression of alpha-smooth muscle (alpha-SM) actin during corneal stromal wound healing.

    Jester JV, Petroll WM, Barry PA, Cavanagh HD 1995 Apr Invest. Ophthalmol. Vis. Sci. 5 36 809-19

    Temporal, 3-dimensional, cellular anatomy of corneal wound tissue.

    Jester JV, Petroll WM, Barry PA, Cavanagh HD 1995 Apr J. Anat. 186 ( Pt 2) 301-11

    Laser and tandem scanning confocal microscopic studies of rabbit corneal wound healing.

    Ichijima H, Jester JV, Petroll WM, Cavanagh HD 1994 Sep-Oct Scanning 5 16 263-8

    In vivo confocal imaging: general principles and applications.

    Petroll WM, Jester JV, Cavanagh HD 1994 May-Jun Scanning 3 16 131-49

    In vivo osmotic pertubation of intercellular fluid channels in the rabbit corneal endothelium.

    Andrews PM, Jester JV, Petroll WM, Barry PA, Ichijima H, Cavanagh HD 1994 May Cornea 3 13 253-8

    The effects of daily wear of rigid gas permeable contact lenses treated with contact lens care solutions containing preservatives on the rabbit cornea.

    Imayasu M, Moriyama T, Ichijima H, Ohashi J, Petroll WM, Jester JV, Cavanagh HD 1994 Jul CLAO J 3 20 183-8

    Corneal keratocytes: in situ and in vitro organization of cytoskeletal contractile proteins.

    Jester JV, Barry PA, Lind GJ, Petroll WM, Garana R, Cavanagh HD 1994 Feb Invest. Ophthalmol. Vis. Sci. 2 35 730-43

    The relation between contact lens oxygen transmissibility and binding of Pseudomonas aeruginosa to the cornea after overnight wear.

    Imayasu M, Petroll WM, Jester JV, Patel SK, Ohashi J, Cavanagh HD 1994 Feb Ophthalmology 2 101 371-88

    In vivo confocal microscopic studies of endothelial wound healing in rabbit cornea.

    Ichijima H, Petroll WM, Jester JV, Barry PA, Andrews PM, Dai M, Cavanagh HD 1993 Sep Cornea 5 12 369-78

    Clinical and diagnostic use of in vivo confocal microscopy in patients with corneal disease.

    Cavanagh HD, Petroll WM, Alizadeh H, He YG, McCulley JP, Jester JV 1993 Oct Ophthalmology 10 100 1444-54

    Noninvasive microscopic evaluation of the intact living nephrotic kidney.

    Pulver M, Petroll WM, Andrews PM 1993 May Lab. Invest. 5 68 592-6

    Three-dimensional imaging of corneal cells using in vivo confocal microscopy.

    Petroll WM, Cavanagh HD, Jester JV 1993 Jun J Microsc Pt 3 170 213-9

    Quantitative analysis of stress fiber orientation during corneal wound contraction.

    Petroll WM, Cavanagh HD, Barry P, Andrews P, Jester JV 1993 Feb J. Cell. Sci. 104 ( Pt 2) 353-63

    Actin filament organization during endothelial wound healing in the rabbit cornea: comparison between transcorneal freeze and mechanical scrape injuries.

    Ichijima H, Petroll WM, Barry PA, Andrews PM, Dai M, Cavanagh HD, Jester JV 1993 Aug Invest. Ophthalmol. Vis. Sci. 9 34 2803-12

    Morphological and biochemical evaluation for rigid gas permeable contact lens extended wear on rabbit corneal epithelium.

    Ichijima H, Ohashi J, Petroll WM, Cavanagh HD 1993 Apr CLAO J 2 19 121-8

    The application of confocal microscopy to the study of living systems.

    Cavanagh HD, Petroll WM, Jester JV 1993 Neurosci Biobehav Rev 4 17 483-98

    Radial keratotomy. III. Relationship between wound gape and corneal curvature in primate eyes.

    Petroll WM, New K, Sachdev M, Cavanagh HD, Jester JV 1992 Nov Invest. Ophthalmol. Vis. Sci. 12 33 3283-91

    Radial keratotomy. II. Role of the myofibroblast in corneal wound contraction.

    Garana RM, Petroll WM, Chen WT, Herman IM, Barry P, Andrews P, Cavanagh HD, Jester JV 1992 Nov Invest. Ophthalmol. Vis. Sci. 12 33 3271-82

    Radial keratotomy. 1. The wound healing process and measurement of incisional gape in two animal models using in vivo confocal microscopy.

    Jester JV, Petroll WM, Feng W, Essepian J, Cavanagh HD 1992 Nov Invest. Ophthalmol. Vis. Sci. 12 33 3255-70

    Confocal microscopic studies of living rabbit cornea treated with benzalkonium chloride.

    Ichijima H, Petroll WM, Jester JV, Cavanagh HD 1992 May Cornea 3 11 221-5

    Effects of increasing Dk with rigid contact lens extended wear on rabbit corneal epithelium using confocal microscopy.

    Ichijima H, Petroll WM, Jester JV, Ohashi J, Cavanagh HD 1992 Jul Cornea 4 11 282-7

    Digital image acquisition in in vivo confocal microscopy.

    Petroll WM, Cavanagh HD, Lemp MA, Andrews PM, Jester JV 1992 Jan J Microsc Pt 1 165 61-9

    Comparison of in vivo and ex vivo cellular structure in rabbit eyes detected by tandem scanning microscopy.

    Jester JV, Petroll WM, Garana RM, Lemp MA, Cavanagh HD 1992 Jan J Microsc Pt 1 165 169-81

    In vivo confocal microscopy in clinical dental research: an initial appraisal.

    Watson TF, Petroll WM, Cavanagh HD, Jester JV 1992 Dec J Dent 6 20 352-8

    Tandem scanning confocal microscopy (TSCM) of normal and ischemic living kidneys.

    Andrews PM, Petroll WM, Cavanagh HD, Jester JV 1991 May Am. J. Anat. 1 191 95-102

    In vivo, real-time confocal imaging.

    Jester JV, Andrews PM, Petroll WM, Lemp MA, Cavanagh HD 1991 May J Electron Microsc Tech 1 18 50-60

    Meibomian gland dysfunction in chronic blepharitis.

    Mathers WD, Shields WJ, Sachdev MS, Petroll WM, Jester JV 1991 Jul Cornea 4 10 277-85

    Meibomian gland morphology and tear osmolarity: changes with Accutane therapy.

    Mathers WD, Shields WJ, Sachdev MS, Petroll WM, Jester JV 1991 Jul Cornea 4 10 286-90

    Relationships between abdominal and diaphragmatic volume displacements.

    Knight H, Petroll WM, Rochester DF 1991 Aug J. Appl. Physiol. 2 71 565-72

    Videofluoroscopic assessment of muscle fiber shortening in the in situ canine diaphragm.

    Knight H, Petroll WM, Adams JM, Shaffer HA, Rochester DF 1990 May J. Appl. Physiol. 5 68 2200-7

    Effect of lower rib cage expansion and diaphragm shortening on the zone of apposition.

    Petroll WM, Knight H, Rochester DF 1990 Feb J. Appl. Physiol. 2 68 484-8

    A model approach to assess diaphragmatic volume displacement.

    Petroll WM, Knight H, Rochester DF 1990 Dec J. Appl. Physiol. 6 69 2175-82

    Contact Petroll Lab

    We want to hear from students and postdoctoral candidates who are interested in joining our lab. Please contact us for more information.

    W. Matthew Petroll, Ph.D. 
    Professor of Ophthalmology Vice Chair, Research
    Chair, Biomedical Engineering Graduate Program
    Email
    X: @PetrollLab

    Mailing Address
    Department of Ophthalmology
    UT Southwestern Medical Center
    5323 Harry Hines Blvd.
    Dallas, TX 75390-9057

     

    Dr. Petroll and his four lab members. l. to r.: Kate Borner (BME grad student), Miguel Miron Mendoza, Ph.D. (Sr. Research Scientist), W. Matthew Petroll, Ph.D., Hajar Hassaniardekani, Ph.D. (Postdoc), and Kara Poole (BME grad student)